Changes in Blood Serum Aldosterone Hormone and Some Mineral Levels in Lipopolysaccharide-Induced Hypothermic Rats

Abstract

In this study, we induced hypothermia in rats by administering LPS (Serotype E. coli: O111:B4; 250 µg/kg, ip). Blood samples were taken via intracardiac puncture at the entrance, deepest point, and exit of the induced hypothermia. In the control group, saline (0.09% NaCl, 0.5 ml/kg ip) was administered to rats, and blood samples were taken at the entrance, deepest point, and exit of hypothermia. It was determined that the administered LPS doses did not change the levels of aldosterone and Na, K, Cl, Ca, and P. As a result of the applications, the deepest point of hypothermia (ΔT: -1.48±0.09 ºC) was reached in an average of 104.46±4.32 minutes. Analysis of blood serum samples revealed that the differences in aldosterone, Na, K, Cl, Ca, and P levels between the LPS-induced hypothermia groups (entry, trough, and exit) compared to the control group were statistically insignificant. Our results indicate that LPS can cause thermoregulatory hypothermia accompanied by fever in rodents.